Application
Western blot analysis of ADAM17 Antibody (N-term)in CEM cell line lysates (35ug/lane). ADAM17 (arrow) was detected using the purified Pab (1:60 dilution).Application
Flow cytometric analysis of CEM cells using ADAM17 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ADAM17 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADAM17; CSVP; TACE; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen=CD156b; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CSVP TACE |
|---|---|
| Alternative Names | ADAM17 CSVP TACE Disintegrin and metalloproteinase domain-containing protein 17 Snake venom-like protease TNF-alpha convertase TNF-alpha-converting enzyme CD_antigen=CD156b Flags: Precursor |
| Molecular Weight(MW) | 93021 Da |
| Function | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway (By similarity) |
| Tissue Specificity | Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 6868 |
|---|
Application
Western blot analysis of ADAM17 Antibody (N-term)in CEM cell line lysates (35ug/lane). ADAM17 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Flow cytometric analysis of CEM cells using ADAM17 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification. |
