Application
Western blot analysis of ADH1B Antibody (Center) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ADH1B Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of ADH1B Antibody (Center)(Cat#AP6738c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ADH1B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADH1B; ADH2; Alcohol dehydrogenase 1B; Alcohol dehydrogenase subunit beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADH2 |
|---|---|
| Alternative Names | ADH1B ADH2 Alcohol dehydrogenase 1B Alcohol dehydrogenase subunit beta |
| Molecular Weight(MW) | 39855 Da |
| Tissue Specificity | This ADH1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216~245 amino acids from the Center region of human ADH1B. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 125 |
|---|
Application
Western blot analysis of ADH1B Antibody (Center) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ADH1B Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of ADH1B Antibody (Center)(Cat#AP6738c) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
