Application
Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) in 293 cell line lysates (35ug/lane). MLLT3(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | AF9 (MLLT3) Antibody (C-term K486) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLLT3; AF9; YEATS3; Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AF9 YEATS3 |
|---|---|
| Alternative Names | MLLT3 AF9 YEATS3 Protein AF-9 ALL1-fused gene from chromosome 9 protein Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein YEATS domain-containing protein 3 |
| Molecular Weight(MW) | 63351 Da |
| Function | Component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA |
| Tissue Specificity | This AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479~509 amino acids from the C-terminal region of human MLLT3. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 4300 |
|---|
Application
Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) in 293 cell line lysates (35ug/lane). MLLT3(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
