Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-AKR1B1 Pab in A375 cell line lysates (35ug/lane).AKR1B1 (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.Application
Confocal immunofluorescent analysis of AKR1B1 Antibody (C-term)(Cat#AP2735b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | AKR1B1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWBIF |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ALDR1 |
|---|---|
| Alternative Names | AKR1B1 ALDR1 Aldose reductase Aldehyde reductase Aldo-keto reductase family 1 member B1 |
| Molecular Weight(MW) | 35853 Da |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies |
| Tissue Specificity | Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 231 |
|---|
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-AKR1B1 Pab in A375 cell line lysates (35ug/lane).AKR1B1 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of AKR1B1 (arrow) using rabbit polyclonal AKR1B1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.
Application
Confocal immunofluorescent analysis of AKR1B1 Antibody (C-term)(Cat#AP2735b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
