Application
Western blot analysis of anti-AKT3 Antibody (N-term) Pab in Hela cell line lysates (35ug/lane). AKT3(arrow) was detected using the purified Pab (1:30 dilution).Application
Western blot analysis of anti-AKT3 Antibody (N-term) Pab (RB11641) in 293 cell line lysates transiently transfected with the AKT3 gene (2ug/lane). AKT3(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with AKT3 Antibody (N-term)(Cat.#7030a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | AKT3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PKBG |
|---|---|
| Alternative Names | AKT3 PKBG RAC-gamma serine/threonine-protein kinase Protein kinase Akt-3 Protein kinase B gamma RAC-PK-gamma STK-2 |
| Molecular Weight(MW) | 55775 Da |
| Function | AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis |
| Tissue Specificity | In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney |
| Cellular Localization | Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation |
| Entrez Gene | 10000 |
|---|
Application
Western blot analysis of anti-AKT3 Antibody (N-term) Pab in Hela cell line lysates (35ug/lane). AKT3(arrow) was detected using the purified Pab (1:30 dilution).
Application
Western blot analysis of anti-AKT3 Antibody (N-term) Pab (RB11641) in 293 cell line lysates transiently transfected with the AKT3 gene (2ug/lane). AKT3(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with AKT3 Antibody (N-term)(Cat.#7030a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
