Application
Dot blot analysis of AMOT Antibody (Phospho Y599) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Product Name | AMOT (Phospho Y599) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMOT; KIAA1071; Angiomotin |
| Modification | p-Y599 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1071 |
|---|---|
| Alternative Names | AMOT KIAA1071 Angiomotin |
| Molecular Weight(MW) | 118085 Da |
| Function | Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions |
| Tissue Specificity | Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta |
| Cellular Localization | Cell junction, tight junction. Note=Localized on the cell surface. May act as a transmembrane protein |
| Entrez Gene | 154796 |
|---|---|
| Nucleotide Accession | NP_001106962.1 |
Application
Dot blot analysis of AMOT Antibody (Phospho Y599) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
