Application
Western blot analysis of ANXA4 antibody (Center) in HepG2 cell line lysates(35ug/lane). ANXA4 (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ANXA4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ANXA4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ANXA4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANXA4; ANX4; Annexin A4; 35-beta calcimedin; Annexin IV; Annexin-4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; P32.5; PP4-X; Placental anticoagulant protein II; Protein II |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ANX4 |
|---|---|
| Alternative Names | ANXA4 ANX4 Annexin A4 35-beta calcimedin Annexin IV Annexin-4 Carbohydrate-binding protein p33/p41 Chromobindin-4 Endonexin I Lipocortin IV P32.5 PP4-X Placental anticoagulant protein II Protein II |
| Molecular Weight(MW) | 35883 Da |
| Function | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis (By similarity) |
| Tissue Specificity | This ANXA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123~153 amino acids from the Center region of human ANXA4. |
| Entrez Gene | 307 |
|---|
Application
Western blot analysis of ANXA4 antibody (Center) in HepG2 cell line lysates(35ug/lane). ANXA4 (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ANXA4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ANXA4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
