Application
AP2B1 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the AP2B1 antibody detected the AP2B1 protein (arrow).| Product Name | AP2B1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP2B1; ADTB2; CLAPB1; AP-2 complex subunit beta; AP105B; Adapter-related protein complex 2 beta subunit; Adaptor protein complex AP-2 subunit beta; Beta-2-adaptin; Beta-adaptin; Clathrin assembly protein complex 2 beta large chain; Plasma membrane adaptor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADTB2 CLAPB1 |
|---|---|
| Alternative Names | AP2B1 ADTB2 CLAPB1 AP-2 complex subunit beta AP105B Adapter-related protein complex 2 beta subunit Adaptor protein complex AP-2 subunit beta Beta-2-adaptin Beta-adaptin Clathrin assembly protein complex 2 beta large chain Plasma membrane adaptor |
| Molecular Weight(MW) | 104553 Da |
| Function | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin- associated sorting proteins (CLASPs) are recognized by their [DE]- X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly |
| Tissue Specificity | This AP2B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-546 amino acids from the Central region of human AP2B1. |
| Cellular Localization | Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV |
| Entrez Gene | 163 |
|---|---|
| Nucleotide Accession | NP_001025177.1 NP_001273.1 |
Application
AP2B1 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the AP2B1 antibody detected the AP2B1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
