Application
Western blot analysis of ARFGAP1 Antibody (C-term) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right). ARFGAP1(arrow) was detected using the purified Pab. Data courtesy of Dr. Dan Cassel, Department of Biology, Technion, Haifa, Israel.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | ARFGAP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARFGAP1; ARF1GAP; ADP-ribosylation factor GTPase-activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1-directed GTPase-activating protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARF1GAP |
|---|---|
| Alternative Names | ARFGAP1 ARF1GAP ADP-ribosylation factor GTPase-activating protein 1 ADP-ribosylation factor 1 GTPase-activating protein ARF1-directed GTPase-activating protein |
| Molecular Weight(MW) | 44668 Da |
| Function | GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity) |
| Tissue Specificity | This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~406 amino acids from the C-terminal region of human ARFGAP1. |
| Cellular Localization | Cytoplasm (By similarity). Golgi apparatus (By similarity). Note=Associates with the Golgi complex (By similarity) |
| Entrez Gene | 55738 |
|---|
Application
Western blot analysis of ARFGAP1 Antibody (C-term) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right). ARFGAP1(arrow) was detected using the purified Pab. Data courtesy of Dr. Dan Cassel, Department of Biology, Technion, Haifa, Israel.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
