Application
Western blot analysis of anti-APG4A Pab in Jurkat cell line lysates (35ug/lane). APG4A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Autophagy APG4A Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
APG4A Antibody (I378) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the APG4A antibody detected the APG4A protein (arrow).| Product Name | ATG4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG4A, AUTL2 |
|---|---|
| Alternative Names | ATG4A APG4A AUTL2 Cysteine protease ATG4A AUT-like 2 cysteine endopeptidase Autophagin-2 Autophagy-related cysteine endopeptidase 2 Autophagy-related protein 4 homolog A |
| Molecular Weight(MW) | 45378 Da |
| Function | Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP |
| Tissue Specificity | Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 115201 |
|---|
Application
Western blot analysis of anti-APG4A Pab in Jurkat cell line lysates (35ug/lane). APG4A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Autophagy APG4A Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
APG4A Antibody (I378) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the APG4A antibody detected the APG4A protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
