Application
Western blot analysis of ATG4D Antibody in MCF-7 cell line lysates (35ug/lane). ATG4D (arrow) was detected using the purified Pab.Application
ATG4D Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATG4D Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
ATG4D Antibody flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATG4D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG4D, AUTL4 |
|---|---|
| Alternative Names | ATG4D APG4D AUTL4 Cysteine protease ATG4D AUT-like 4 cysteine endopeptidase Autophagin-4 Autophagy-related cysteine endopeptidase 4 Autophagy-related protein 4 homolog D |
| Molecular Weight(MW) | 52922 Da |
| Function | Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes |
| Tissue Specificity | Mainly expressed in skeletal muscle and, to a lower extent, in testis |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 84971 |
|---|
Application
Western blot analysis of ATG4D Antibody in MCF-7 cell line lysates (35ug/lane). ATG4D (arrow) was detected using the purified Pab.
Application
ATG4D Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATG4D Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ATG4D Antibody flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
