Application
A. Western blot analysis on Raji cell lysates using anti-Phospho-B-Myb (pT487) RabMAb (Cat. #AJ1103a), 1:1000 dilution. Cells were either (A) untreated (B) treated with TPA + IGFApplication
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-B-Myb RabMAb (Cat. #AJ1103a)Application
C. Immunofluorescent staining of HeLa cells using Phospho-anti-B-Myb RabMAb(Cat. #AJ1103a).| Product Name | B-Myb Antibody Phospho (pT487) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T487 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2204Y |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BMYB |
|---|---|
| Alternative Names | MYBL2 BMYB Myb-related protein B B-Myb |
| Molecular Weight(MW) | 78764 Da |
| Function | Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding threonine 487 of human B-Myb was used as an immunogen. This antibody detects B-Myb phosphorlyated on threonine 487. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4605 |
|---|
Application
A. Western blot analysis on Raji cell lysates using anti-Phospho-B-Myb (pT487) RabMAb (Cat. #AJ1103a), 1:1000 dilution. Cells were either (A) untreated (B) treated with TPA + IGF
Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti-B-Myb RabMAb (Cat. #AJ1103a)
Application
C. Immunofluorescent staining of HeLa cells using Phospho-anti-B-Myb RabMAb(Cat. #AJ1103a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
