Application
Western blot analysis of BF Antibody (Center) (Cat.# AP7357c) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). BF (arrow) was detected using the purified Pab.Application
BF Antibody (Center) (RB18685) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | BF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFB; BF; BFD; Complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; PBF2; Properdin factor B; Complement factor B Ba fragment; Complement factor B Bb fragment |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BF BFD |
|---|---|
| Alternative Names | CFB BF BFD Complement factor B C3/C5 convertase Glycine-rich beta glycoprotein PBF2 Properdin factor B Complement factor B Ba fragment Complement factor B Bb fragment |
| Molecular Weight(MW) | 85533 Da |
| Function | Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B- lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes Ba inhibits the proliferation of preactivated B-lymphocytes |
| Tissue Specificity | This BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401~431 amino acids from the Center region of human BF. |
| Cellular Localization | Secreted. |
| Entrez Gene | 629 |
|---|
Application
Western blot analysis of BF Antibody (Center) (Cat.# AP7357c) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). BF (arrow) was detected using the purified Pab.
Application
BF Antibody (Center) (RB18685) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
