Application
BHLH3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the BHLH3 antibody detected the BHLH3 protein (arrow).Application
BHLH3 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the BHLH3 antibody detected BHLH3 protein (arrow).Application
BHLH3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term)(Cat#AP5748a). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.| Product Name | BHLH3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BHLHE41; BHLHB3; DEC2; SHARP1; Class E basic helix-loop-helix protein 41; Class B basic helix-loop-helix protein 3; Differentially expressed in chondrocytes protein 2; Enhancer-of-split and hairy-related protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB3 DEC2 SHARP1 |
|---|---|
| Alternative Names | BHLHE41 BHLHB3 DEC2 SHARP1 Class E basic helix-loop-helix protein 41 Class B basic helix-loop-helix protein 3 Differentially expressed in chondrocytes protein 2 Enhancer-of-split and hairy-related protein 1 |
| Molecular Weight(MW) | 50498 Da |
| Function | May be a transcriptional repressor that represses both basal and activated transcription |
| Tissue Specificity | Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney |
| Cellular Localization | Nucleus (By similarity). |
Application
BHLH3 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the BHLH3 antibody detected the BHLH3 protein (arrow).
Application
BHLH3 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the BHLH3 antibody detected BHLH3 protein (arrow).
Application
BHLH3 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term)(Cat#AP5748a). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
