Application
BHLHA15 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the BHLHA15 antibody detected the BHLHA15 protein (arrow).Application
BHLHA15 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the BHLHA15 antibody detected the BHLHA15 protein (arrow).Application
BHLHA15 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BHLHA15 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BHLHA15; BHLHB8; MIST1; Class A basic helix-loop-helix protein 15; Class B basic helix-loop-helix protein 8; Muscle, intestine and stomach expression 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB8 MIST1 |
|---|---|
| Alternative Names | BHLHA15 BHLHB8 MIST1 Class A basic helix-loop-helix protein 15 Class B basic helix-loop-helix protein 8 Muscle intestine and stomach expression 1 |
| Molecular Weight(MW) | 20818 Da |
| Function | Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3' (By similarity) |
| Tissue Specificity | Expressed in brain, liver, spleen and skeletal muscle |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 168620 |
|---|---|
| Nucleotide Accession | NP_803238.1 |
Application
BHLHA15 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the BHLHA15 antibody detected the BHLHA15 protein (arrow).
Application
BHLHA15 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the BHLHA15 antibody detected the BHLHA15 protein (arrow).
Application
BHLHA15 Antibody (C-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM227632