Application
Western blot analysis of BLNK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). BLNK (arrow) was detected using the purified Pab.Application
BLNK Antibody (Center) (RB18883) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BLNK Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
BLNK Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BLNK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BLNK; BASH; SLP65; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BASH SLP65 |
|---|---|
| Alternative Names | BLNK BASH SLP65 B-cell linker protein B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein Cytoplasmic adapter protein Src homology 2 domain-containing leukocyte protein of 65 kDa |
| Molecular Weight(MW) | 50466 Da |
| Function | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR- mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis |
| Tissue Specificity | Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon |
| Cellular Localization | Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction |
| Entrez Gene | 29760 |
|---|
Application
Western blot analysis of BLNK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). BLNK (arrow) was detected using the purified Pab.
Application
BLNK Antibody (Center) (RB18883) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BLNK Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
BLNK Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
