Application
Western blot analysis of C5 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). C5 (arrow) was detected using the purified Pab.(2ug/ml)Application
Formalin-fixed and paraffin-embedded human breast carcinoma with C5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using C5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | C5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C5; CPAMD4; Complement C5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; Complement C5 beta chain; Complement C5 alpha chain; C5a anaphylatoxin; Complement C5 alpha' chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPAMD4 |
|---|---|
| Alternative Names | C5 CPAMD4 Complement C5 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 Complement C5 beta chain Complement C5 alpha chain C5a anaphylatoxin Complement C5 alpha' chain |
| Molecular Weight(MW) | 188305 Da |
| Function | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled |
| Tissue Specificity | This C5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260~289 amino acids from the N-terminal region of human C5. |
| Cellular Localization | Secreted. |
| Entrez Gene | 727 |
|---|
Application
Western blot analysis of C5 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). C5 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with C5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using C5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
