Application
Western blot analysis of CAMKK2 (arrow) using rabbit polyclonal CAMKK2 Antibody (N-term G67) . 293 cell lysates (2 ug/lane) either nontransfected (c) or transiently transfected with the CAMKK2 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CAMKK2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CAMKK2 Antibody (N-term G67) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAMKK2; CAMKKB; KIAA0787; Calcium/calmodulin-dependent protein kinase kinase 2; Calcium/calmodulin-dependent protein kinase kinase beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAMKKB KIAA0787 |
|---|---|
| Alternative Names | CAMKK2 CAMKKB KIAA0787 Calcium/calmodulin-dependent protein kinase kinase 2 Calcium/calmodulin-dependent protein kinase kinase beta |
| Molecular Weight(MW) | 64746 Da |
| Function | Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin- binding domain are inactive. Efficiently phosphorylates 5'-AMP- activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching |
| Tissue Specificity | Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung |
| Cellular Localization | Nucleus (By similarity). Cytoplasm. Cell projection. Note=Predominantly nuclear in unstimulated cells (By similarity). Found in the cytoplasm and neurites after forskolin induction |
| Entrez Gene | 10645 |
|---|
Application
Western blot analysis of CAMKK2 (arrow) using rabbit polyclonal CAMKK2 Antibody (N-term G67) . 293 cell lysates (2 ug/lane) either nontransfected (c) or transiently transfected with the CAMKK2 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CAMKK2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
