Application
Western blot analysis of CASP3 (Asp175) Antibody in CEM cell line lysates (35ug/lane). CASP3 (arrow) was detected using the purified Pab.Application
CASP3(Asp175) Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP3(Asp175) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.Application
CASP3(Asp175) Antibody flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CASP3(Asp175) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP3; CPP32; Caspase-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPP32 |
|---|---|
| Alternative Names | CASP3 CPP32 Caspase-3 Apopain Cysteine protease CPP32 Protein Yama SREBP cleavage activity 1 Caspase-3 subunit p17 Caspase-3 subunit p12 |
| Molecular Weight(MW) | 31608 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage |
| Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system |
| Cellular Localization | Cytoplasm. |
Application
Western blot analysis of CASP3 (Asp175) Antibody in CEM cell line lysates (35ug/lane). CASP3 (arrow) was detected using the purified Pab.
Application
CASP3(Asp175) Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP3(Asp175) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.
Application
CASP3(Asp175) Antibody flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
