Application
A. Western blot analysis on Jurkat cell lysates using anti-Caspase-3 RabMAb (Cat. #AJ1131b). Cells were either (A) untreated or (B) treated with Camptothecin.Application
B. Immunofluorescent staining of untreated HeLa cells using anti-Caspase-3 RabMAb (Cat. #AJ1131b).Application
C. Immunofluorescent staining of HeLa cells treated with staurosporine using anti-Caspase-3 RabMAb (Cat. #AJ1131b) showing activation of Caspase-3 in these cells.Application
D. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Caspase-3 RabMAb (Cat. #AJ1131b).| Product Name | Caspase-3 Antibody (Active) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP3, CPP32, Caspase-3 subunit p12, Yama protein;SREBP cleavage activity 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E83-77 |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPP32 |
|---|---|
| Alternative Names | CASP3 CPP32 Caspase-3 subunit p12 Yama protein SREBP cleavage activity 1 |
| Molecular Weight(MW) | 31608 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage |
| Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 836 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Caspase-3 RabMAb (Cat. #AJ1131b). Cells were either (A) untreated or (B) treated with Camptothecin.
Application
B. Immunofluorescent staining of untreated HeLa cells using anti-Caspase-3 RabMAb (Cat. #AJ1131b).
Application
C. Immunofluorescent staining of HeLa cells treated with staurosporine using anti-Caspase-3 RabMAb (Cat. #AJ1131b) showing activation of Caspase-3 in these cells.
Application
D. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Caspase-3 RabMAb (Cat. #AJ1131b).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
