Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-CD3 zeta (pY142) RabMAb (Cat. #AJ1162b), 1:2,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Product Name | CD3-zeta Antibody Phospho (pY142) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y142 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP265(2)Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD3Z, T3Z, TCRZ |
|---|---|
| Alternative Names | CD247 CD3Z T3Z TCRZ T-cell surface glycoprotein CD3 zeta chain T-cell receptor T3 zeta chain |
| Molecular Weight(MW) | 18696 Da |
| Function | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding tyrosine 142 of human CD3 zeta was used as an immunogen. This antibody detects CD3 zeta phosphorylated on tyrosine 142. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 919 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho-CD3 zeta (pY142) RabMAb (Cat. #AJ1162b), 1:2,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
