Application
Western blot analysis of CD3G Antibody (N-term) in HL60 cell line lysates (35ug/lane). CD3G (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD3G antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HL-60 cells using CD3G Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD3G Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD3G; T3G; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD_antigen=CD3g; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | T3G |
|---|---|
| Alternative Names | CD3G T3G T-cell surface glycoprotein CD3 gamma chain T-cell receptor T3 gamma chain CD_antigen=CD3g Flags: Precursor |
| Molecular Weight(MW) | 20469 Da |
| Function | The CD3 complex mediates signal transduction. |
| Tissue Specificity | This CD3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55~84 amino acids from the N-terminal region of human CD3G. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 917 |
|---|
Application
Western blot analysis of CD3G Antibody (N-term) in HL60 cell line lysates (35ug/lane). CD3G (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CD3G antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HL-60 cells using CD3G Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification. |
