Application
Western blot analysis of anti-CD3Z Antibody (N-term) (RB13817) in Ramos cell line lysates (35ug/lane). CD3Z(arrow) was detected using the purified Pab (1:60 dilution).Application
Flow cytometric analysis of Ramos cells using CD3Z Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD3Z Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD_antigen=CD247; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD3Z T3Z TCRZ |
|---|---|
| Alternative Names | CD247 CD3Z T3Z TCRZ T-cell surface glycoprotein CD3 zeta chain T-cell receptor T3 zeta chain CD_antigen=CD247 Flags: Precursor |
| Molecular Weight(MW) | 18696 Da |
| Function | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering |
| Tissue Specificity | This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10~39 amino acids from the N-terminal region of human CD3Z. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 919 |
|---|
Application
Western blot analysis of anti-CD3Z Antibody (N-term) (RB13817) in Ramos cell line lysates (35ug/lane). CD3Z(arrow) was detected using the purified Pab (1:60 dilution).
Application
Flow cytometric analysis of Ramos cells using CD3Z Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
