Application
A. Western blot analysis on Jurkat cell lysate using anti-CD45 RabMAb (Cat. #AJ1164b).Application
B. Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45 RabMAb (red) (Cat. #AJ1164b) and a rabbit IgG (negative) (green).Application
C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-CD45 RabMAb (Cat. #AJ1164b)| Product Name | CD45 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPRC, CD45, Leukocyte common antigen, T200 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP322Y |
| Host Species | Rabbit |
| Tested Applications | WBFCIHC |
| WB:1:1000~10000 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD45 |
|---|---|
| Alternative Names | PTPRC CD45 Leukocyte common antigen T200 |
| Molecular Weight(MW) | 147254 Da |
| Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-terminal domain of human CD45 was used as immunogen. |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts |
| Entrez Gene | 5788 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-CD45 RabMAb (Cat. #AJ1164b).
Application
B. Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45 RabMAb (red) (Cat. #AJ1164b) and a rabbit IgG (negative) (green).
Application
C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-CD45 RabMAb (Cat. #AJ1164b)| Application Notes | WB:1:1000~10000 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM229248