Application
Western blot analysis of anti-CD8B1 Pab in K562 cell line lysates (35ug/lane). CD8B1(arrow) was detected using the purified Pab (1:60 dilution).Application
Flow cytometric analysis of jurkat cells using CD8B Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD8B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD8B; CD8B1; T-cell surface glycoprotein CD8 beta chain; CD_antigen=CD8b; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD8B1 |
|---|---|
| Alternative Names | CD8B CD8B1 T-cell surface glycoprotein CD8 beta chain CD_antigen=CD8b Flags: Precursor |
| Molecular Weight(MW) | 23722 Da |
| Function | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing |
| Tissue Specificity | Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein (Probable) Isoform 3: Secreted (Probable). Isoform 5: Cell membrane; Single-pass type I membrane protein (Probable) Isoform 7: Secreted (Probable). |
| Entrez Gene | 926 |
|---|
Application
Western blot analysis of anti-CD8B1 Pab in K562 cell line lysates (35ug/lane). CD8B1(arrow) was detected using the purified Pab (1:60 dilution).
Application
Flow cytometric analysis of jurkat cells using CD8B Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
