Application
Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).Application
Immunofluorescence analysis of anti-CD9 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Application
Flow cytometric analysis of jurkat cells using CD9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD9 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD9; MIC3; TSPAN29; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; Tetraspanin-29; p24; CD_antigen=CD9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIC3 TSPAN29 |
|---|---|
| Alternative Names | CD9 MIC3 TSPAN29 CD9 antigen 5H9 antigen Cell growth-inhibiting gene 2 protein Leukocyte antigen MIC3 Motility-related protein Tetraspanin-29 p24 CD_antigen=CD9 |
| Molecular Weight(MW) | 25416 Da |
| Function | Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion |
| Tissue Specificity | Expressed by a variety of hematopoietic and epithelial cells |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| Entrez Gene | 928 |
|---|
Application
Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
Application
Immunofluorescence analysis of anti-CD9 Antibody (Center) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Application
Flow cytometric analysis of jurkat cells using CD9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
