Application
Western blot analysis of anti-CDH3 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDH3(arrow) was detected using the purified Pab (1:60 dilution).Western blot analysis of CDH3 (arrow) using rabbit polyclonal CDH3 Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with CDH3 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CDH3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDH3; CDHP; Cadherin-3; Placental cadherin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDHP |
|---|---|
| Alternative Names | CDH3 CDHP Cadherin-3 Placental cadherin |
| Molecular Weight(MW) | 91418 Da |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types |
| Tissue Specificity | Expressed in some normal epithelial tissues and in some carcinoma cell lines |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 1001 |
|---|
Application
Western blot analysis of anti-CDH3 Antibody (C-term) in K562 cell line lysates (35ug/lane). CDH3(arrow) was detected using the purified Pab (1:60 dilution).Western blot analysis of CDH3 (arrow) using rabbit polyclonal CDH3 Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with CDH3 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification. |
