Application
Western blot analysis of CDK3(arrow) using rabbit polyclonal CDK3 Antibody (N-term Y19) (Cat. #). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK3 Antibody (N-term Y19), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using CDK3 Antibody (N-term Y19)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDK3 Antibody (N-term Y19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK3; CDKN3; Cyclin-dependent kinase 3; Cell division protein kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDKN3 |
|---|---|
| Alternative Names | CDK3 CDKN3 Cyclin-dependent kinase 3 Cell division protein kinase 3 |
| Molecular Weight(MW) | 35046 Da |
| Function | Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0- G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1- independent manner |
| Tissue Specificity | Expressed in cancer cell lines and glioblastoma tissue |
| Entrez Gene | 1018 |
|---|
Application
Western blot analysis of CDK3(arrow) using rabbit polyclonal CDK3 Antibody (N-term Y19) (Cat. #). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK3 Antibody (N-term Y19), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using CDK3 Antibody (N-term Y19)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
