Application
Western blot analysis of CDKN2B (arrow) using rabbit polyclonal CDKN2B Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2B gene.Application
CDKN2B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDKN2B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of CDKN2B Antibody (C-term)(Cat#AP12630b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
CDKN2B Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDKN2B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDKN2B; MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; p14-INK4b; p15-INK4b |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MTS2 |
|---|---|
| Alternative Names | CDKN2B MTS2 Cyclin-dependent kinase 4 inhibitor B Multiple tumor suppressor 2 p14-INK4b p15-INK4b |
| Molecular Weight(MW) | 14722 Da |
| Function | Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest |
| Tissue Specificity | Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines |
| Cellular Localization | Cytoplasm. Note=Also found in the nucleus. |
| Entrez Gene | 1030 |
|---|---|
| Nucleotide Accession | NP_511042.1 NP_004927.2 |
Application
Western blot analysis of CDKN2B (arrow) using rabbit polyclonal CDKN2B Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2B gene.
Application
CDKN2B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDKN2B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of CDKN2B Antibody (C-term)(Cat#AP12630b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
CDKN2B Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
