Application
Western blot analysis of CENPT Antibody (C-term) in T47D cell line lysates (35ug/lane). CENPT (arrow) was detected using the purified Pab;Application
Western blot analysis of CENPT Antibody (C-term) in Neuro-2a mouse cell line lysates (35ug/lane). CENPT (arrow) was detected using the purified Pab.| Product Name | CENPT Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPT; C16orf56; ICEN22; Centromere protein T; Interphase centromere complex protein 22 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C16orf56 ICEN22 |
|---|---|
| Alternative Names | CENPT C16orf56 ICEN22 Centromere protein T Interphase centromere complex protein 22 |
| Molecular Weight(MW) | 60423 Da |
| Function | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome- associated complex that binds specifically to histone H3- containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis |
| Tissue Specificity | This CENPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CENPT. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus |
| Entrez Gene | 80152 |
|---|
Application
Western blot analysis of CENPT Antibody (C-term) in T47D cell line lysates (35ug/lane). CENPT (arrow) was detected using the purified Pab;
Application
Western blot analysis of CENPT Antibody (C-term) in Neuro-2a mouse cell line lysates (35ug/lane). CENPT (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
