Application
Western blot analysis of CIRBP Antibody (C-term) in Y79 cell line lysates (35ug/lane). CIRBP (arrow) was detected using the purified Pab.Application
CIRBP Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CIRBP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of CIRBP (arrow) using rabbit polyclonal CIRBP Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CIRBP gene.| Product Name | CIRBP Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIRBP; A18HNRNP; CIRP; Cold-inducible RNA-binding protein; A18 hnRNP; Glycine-rich RNA-binding protein CIRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | A18HNRNP CIRP |
|---|---|
| Alternative Names | CIRBP A18HNRNP CIRP Cold-inducible RNA-binding protein A18 hnRNP Glycine-rich RNA-binding protein CIRP |
| Molecular Weight(MW) | 18648 Da |
| Function | Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold- induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus, nucleoplasm. Cytoplasm. Note=Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins (By similarity) |
| Entrez Gene | 1153 |
|---|
Application
Western blot analysis of CIRBP Antibody (C-term) in Y79 cell line lysates (35ug/lane). CIRBP (arrow) was detected using the purified Pab.
Application
CIRBP Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CIRBP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of CIRBP (arrow) using rabbit polyclonal CIRBP Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CIRBP gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
