Application
Western blot analysis of CNOT4 Antibody in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CNOT4 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CNOT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CNOT4; NOT4; CCR4-NOT transcription complex subunit 4; CCR4-associated factor 4; E3 ubiquitin-protein ligase CNOT4; Potential transcriptional repressor NOT4Hp |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NOT4 |
|---|---|
| Alternative Names | CNOT4 NOT4 CCR4-NOT transcription complex subunit 4 CCR4-associated factor 4 E3 ubiquitin-protein ligase CNOT4 Potential transcriptional repressor NOT4Hp |
| Molecular Weight(MW) | 63510 Da |
| Function | Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex |
| Tissue Specificity | This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein. |
| Cellular Localization | Cytoplasm (Probable). Nucleus (Probable). |
| Entrez Gene | 4850 |
|---|
Application
Western blot analysis of CNOT4 Antibody in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CNOT4 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
