Application
A. Western blot analysis on (A) HT-29, (B) HeLa, (C) SKBR-3 and (D) 293T cell lysates using anti-COPS3 RabMAb (Cat. #AJ1193a), dilution: 1:20,000.Application
B. Immunofluorescent staining of HeLa cells using anti-COPS3 RabMAb (Cat. #AJ1193a)| Product Name | COPS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COPS3, CSN3, COP9 signalosome complex subunit 3, JAB1-containing signalosome subunit 3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3127 |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CSN3 |
|---|---|
| Alternative Names | COPS3 CSN3 COP9 signalosome complex subunit 3 JAB1-containing signalosome subunit 3 |
| Molecular Weight(MW) | 47873 Da |
| Function | Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively |
| Tissue Specificity | Widely expressed. Expressed at high level in heart and skeletal muscle |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 8533 |
|---|
Application
A. Western blot analysis on (A) HT-29, (B) HeLa, (C) SKBR-3 and (D) 293T cell lysates using anti-COPS3 RabMAb (Cat. #AJ1193a), dilution: 1:20,000.
Application
B. Immunofluorescent staining of HeLa cells using anti-COPS3 RabMAb (Cat. #AJ1193a)| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
