Application
COXII antibody(Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COXII antibody(Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
COXII Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of COXII Antibody (Center)(Cat#AP10129c) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
COXII Antibody (Center) western blot analysis in Jurkat,MCF-7,A549 cell line lysates (35ug/lane).This demonstrates the COXII antibody detected the COXII protein (arrow).| Product Name | COXII Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MT-CO2; COII; COXII; MTCO2; Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCFCIFWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | COII COXII MTCO2 |
|---|---|
| Alternative Names | MT-CO2 COII COXII MTCO2 Cytochrome c oxidase subunit 2 Cytochrome c oxidase polypeptide II |
| Molecular Weight(MW) | 25565 Da |
| Function | Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1 |
| Tissue Specificity | This COXII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human COXII. |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein |
| Entrez Gene | 4513 |
|---|---|
| Nucleotide Accession | YP_003024029.1 |
Application
COXII antibody(Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COXII antibody(Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
COXII Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of COXII Antibody (Center)(Cat#AP10129c) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
COXII Antibody (Center) western blot analysis in Jurkat,MCF-7,A549 cell line lysates (35ug/lane).This demonstrates the COXII antibody detected the COXII protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
