Application
COXI Antibody (Center) western blot analysis in MCF-7 cell line lysates.This demonstrates the COXI antibody detected the COXI protein (arrow)(Kindly provided by Dr. John Wu).Application
Western blot analysis of COXI Antibody (Center) in HL-60 cell line lysates (35ug/lane). COXI (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of COXI Antibody (Center)(Cat#AP9684c) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | COXI Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MT-CO1; COI; COXI; MTCO1; Cytochrome c oxidase subunit 1; Cytochrome c oxidase polypeptide I |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | COI COXI MTCO1 |
|---|---|
| Alternative Names | MT-CO1 COI COXI MTCO1 Cytochrome c oxidase subunit 1 Cytochrome c oxidase polypeptide I |
| Molecular Weight(MW) | 57041 Da |
| Function | Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1- 3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B |
| Tissue Specificity | This COXI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202~232 amino acids from the Central region of human COXI. |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein |
| Entrez Gene | 4512 |
|---|
Application
COXI Antibody (Center) western blot analysis in MCF-7 cell line lysates.This demonstrates the COXI antibody detected the COXI protein (arrow)(Kindly provided by Dr. John Wu).
Application
Western blot analysis of COXI Antibody (Center) in HL-60 cell line lysates (35ug/lane). COXI (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of COXI Antibody (Center)(Cat#AP9684c) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
