Application
Confocal immunofluorescent analysis of DDC Antibody (N-term)(Cat#AP5536a) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
DDC Antibody (N-term) western blot analysis in mouse liver,kidney tissue lysates (15ug/lane).This demonstrates the DDC antibody detected DDC protein (arrow).Application
DDC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DDC Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DDC Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDC; AADC; Aromatic-L-amino-acid decarboxylase; DOPA decarboxylase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHCFC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AADC |
|---|---|
| Alternative Names | DDC AADC Aromatic-L-amino-acid decarboxylase DOPA decarboxylase |
| Molecular Weight(MW) | 53926 Da |
| Function | Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine |
| Tissue Specificity | This DDC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from the N-terminal region of human DDC. |
| Entrez Gene | 1644 |
|---|---|
| Nucleotide Accession | NP_000781.1 |
Application
Confocal immunofluorescent analysis of DDC Antibody (N-term)(Cat#AP5536a) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
DDC Antibody (N-term) western blot analysis in mouse liver,kidney tissue lysates (15ug/lane).This demonstrates the DDC antibody detected DDC protein (arrow).
Application
DDC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DDC Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
