Application
Western blot analysis of DDX5 antibody (Center) (Cat.# AP7459c) in Romas cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain with DDX5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | DDX5 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDX5; G17P1; HELR; HLR1; Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | G17P1 HELR HLR1 |
|---|---|
| Alternative Names | DDX5 G17P1 HELR HLR1 Probable ATP-dependent RNA helicase DDX5 DEAD box protein 5 RNA helicase p68 |
| Molecular Weight(MW) | 69148 Da |
| Function | Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10 The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1 |
| Tissue Specificity | This DDX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313~342 amino acids from the Center region of human DDX5. |
| Cellular Localization | Nucleus, nucleolus. |
| Entrez Gene | 1655 |
|---|
Application
Western blot analysis of DDX5 antibody (Center) (Cat.# AP7459c) in Romas cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain with DDX5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
