Application
DEAF1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DEAF1 antibody detected the DEAF1 protein (arrow).| Product Name | DEAF1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DEAF1; SPN; ZMYND5; Deformed epidermal autoregulatory factor 1 homolog; Nuclear DEAF-1-related transcriptional regulator; Suppressin; Zinc finger MYND domain-containing protein 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SPN ZMYND5 |
|---|---|
| Alternative Names | DEAF1 SPN ZMYND5 Deformed epidermal autoregulatory factor 1 homolog Nuclear DEAF-1-related transcriptional regulator Suppressin Zinc finger MYND domain-containing protein 5 |
| Molecular Weight(MW) | 59327 Da |
| Function | Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'- AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein- protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase Required for neural tube closure and skeletal patterning Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm |
| Tissue Specificity | Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls |
| Cellular Localization | Isoform 1: Nucleus. Cytoplasm. Note=Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270% Isoform 3: Secreted. Note=Secreted in some cell types |
| Entrez Gene | 10522 |
|---|---|
| Nucleotide Accession | NP_066288.2 |
Application
DEAF1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DEAF1 antibody detected the DEAF1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
