Application
A. Western blot analysis on K562 cell lysate using anti- PRKD/CDNA-PK RabMAb (Cat. #AJ1242a), dilution 1:1,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PRKD/CDNA-PK Rab MAb (Cat. #AJ1242a).Application
C. Immunofluorescent staining of HeLa cells using anti-PRKD/CDNA-PK RabMAb (Cat. #AJ1242a).| Product Name | DNA-PK/PRKDC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKDC, HYRC, HYRC1, DNA-dependent protein kinase catalytic subunit, DNPK1;p460 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y393 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HYRC HYRC1 |
|---|---|
| Alternative Names | PRKDC HYRC HYRC1 DNA-dependent protein kinase catalytic subunit DNPK1 p460 |
| Molecular Weight(MW) | 469089 Da |
| Function | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2 Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-term of human DNA-PK was used as immunogen. This antibody may detect splice isoform 2. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. |
| Entrez Gene | 5591 |
|---|
Application
A. Western blot analysis on K562 cell lysate using anti- PRKD/CDNA-PK RabMAb (Cat. #AJ1242a), dilution 1:1,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PRKD/CDNA-PK Rab MAb (Cat. #AJ1242a).
Application
C. Immunofluorescent staining of HeLa cells using anti-PRKD/CDNA-PK RabMAb (Cat. #AJ1242a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
