Application
Western blot analysis of DNMT1 Antibody (S1105) (Cat.# AP1032b) in MDA-MB435 cell line lysates (35ug/lane). DNMT1 (arrow) was detected using the purified Pab.| Product Name | DNMT1 Antibody (S1105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNMT1; AIM; CXXC9; DNMT; DNA (cytosine-5)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; MCMT |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AIM CXXC9 DNMT |
|---|---|
| Alternative Names | DNMT1 AIM CXXC9 DNMT DNA (cytosine-5)-methyltransferase 1 CXXC-type zinc finger protein 9 DNA methyltransferase HsaI MCMT |
| Molecular Weight(MW) | 183165 Da |
| Function | Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9 |
| Tissue Specificity | Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1 |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of DNMT1 Antibody (S1105) (Cat.# AP1032b) in MDA-MB435 cell line lysates (35ug/lane). DNMT1 (arrow) was detected using the purified Pab.| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
