Application
Western blot analysis of DPPA2 Antibody in Hela cell line lysates (35ug/lane). DPPA2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human placenta with DPPA2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of DPPA2 Antibody with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Application
Flow cytometric analysis of hela cells using DPPA2 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DPPA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPPA2; PESCRG1; Developmental pluripotency-associated protein 2; Pluripotent embryonic stem cell-related gene 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PESCRG1 |
|---|---|
| Alternative Names | DPPA2 PESCRG1 Developmental pluripotency-associated protein 2 Pluripotent embryonic stem cell-related gene 1 protein |
| Molecular Weight(MW) | 33784 Da |
| Function | Binds to target gene promoters, including NKX2-5 and SYCE1, but not GATA4, and may be involved in the maintenance of the active epigenetic status of these genes (By similarity) |
| Tissue Specificity | Expressed in embryonic stem cells. No expression is seen in 5 months embryo, mesenchymal stem cells, embryonic fibrocytes and adult tissues |
| Cellular Localization | Nucleus. |
| Entrez Gene | 151871 |
|---|
Application
Western blot analysis of DPPA2 Antibody in Hela cell line lysates (35ug/lane). DPPA2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human placenta with DPPA2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of DPPA2 Antibody with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Application
Flow cytometric analysis of hela cells using DPPA2 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
