Application
Western blot analysis of DUSP3 (arrow) using rabbit polyclonal DUSP3 Antibody (C171) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP3 gene.Application
DUSP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUSP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
DUSP3 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DUSP3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DUSP3; VHR; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VHR |
|---|---|
| Alternative Names | DUSP3 VHR Dual specificity protein phosphatase 3 Dual specificity protein phosphatase VHR Vaccinia H1-related phosphatase |
| Molecular Weight(MW) | 20478 Da |
| Function | Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2 |
| Tissue Specificity | This DUSP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156~185 amino acids from the C-terminal region of human DUSP3. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1845 |
|---|
Application
Western blot analysis of DUSP3 (arrow) using rabbit polyclonal DUSP3 Antibody (C171) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP3 gene.
Application
DUSP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUSP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
DUSP3 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
