Application
Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293T cell line lysate. E2F1 Antibody (H357) (arrow) was detected using the purified Pab (1:60/250 dilution).| Product Name | E2F1 Antibody (H357) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F1; RBBP3; Transcription factor E2F1; PBR3; PRB-binding protein E2F-1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50~100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RBBP3 |
|---|---|
| Alternative Names | E2F1 RBBP3 Transcription factor E2F1 PBR3 PRB-binding protein E2F-1 Retinoblastoma-associated protein 1 Retinoblastoma-binding protein 3 |
| Molecular Weight(MW) | 46920 Da |
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis |
| Tissue Specificity | This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding H357 of human E2F1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1869 |
|---|
Application
Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293T cell line lysate. E2F1 Antibody (H357) (arrow) was detected using the purified Pab (1:60/250 dilution).| Application Notes | WB:1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
