Application
Western blot analysis of EBP1 Antibody (Center) in MCF7,A2058 cell line lysates(35ug/lane). EBP1 (arrow) was detected using the purified Pab.Application
EBP1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EBP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EBP1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PA2G4; EBP1; Proliferation-associated protein 2G4; Cell cycle protein p38-2G4 homolog; ErbB3-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EBP1 |
|---|---|
| Alternative Names | PA2G4 EBP1 Proliferation-associated protein 2G4 Cell cycle protein p38-2G4 homolog ErbB3-binding protein 1 |
| Molecular Weight(MW) | 43787 Da |
| Function | May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity) |
| Tissue Specificity | Expressed in several cell lines tested, including primary and transformed cell lines |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. Note=Tranlocates to the nucleus upon treatment with HRG |
| Entrez Gene | 5036 |
|---|
Application
Western blot analysis of EBP1 Antibody (Center) in MCF7,A2058 cell line lysates(35ug/lane). EBP1 (arrow) was detected using the purified Pab.
Application
EBP1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EBP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
