Application
A. Western blot analysis on (A) 293 (B) HeLa (C) A431 (D) NIH3T3 cell lysate using anti-eEF2 RabMAb (Cat. #AJ1250c)Application
B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-eEF2 RabMAb (Cat. #AJ1250c).| Product Name | eEF2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP880Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EF2 |
|---|---|
| Molecular Weight(MW) | 95338 Da |
| Function | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome |
| Tissue Specificity | A synthetic peptide corresponding to residues on the C-terminus of human eEF2 was used as an immunogen. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1938 |
|---|
Application
A. Western blot analysis on (A) 293 (B) HeLa (C) A431 (D) NIH3T3 cell lysate using anti-eEF2 RabMAb (Cat. #AJ1250c)
Application
B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-eEF2 RabMAb (Cat. #AJ1250c).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
