Application
Western blot analysis of EEF2 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35ug/lane). EEF2 (arrow) was detected using the purified Pab.Application
Western blot analysis of EEF2 Antibody (N-term) in Y79 cell line lysates (35ug/lane). EEF2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with EEF2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
EEF2 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | EEF2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EF2 |
|---|---|
| Alternative Names | EEF2 EF2 Elongation factor 2 |
| Molecular Weight(MW) | 95338 Da |
| Function | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome |
| Tissue Specificity | This EEF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45~75 amino acids from the N-terminal region of human EEF2. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1938 |
|---|
Application
Western blot analysis of EEF2 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35ug/lane). EEF2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of EEF2 Antibody (N-term) in Y79 cell line lysates (35ug/lane). EEF2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with EEF2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
EEF2 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
