Application
Western blot analysis on 293 cell lysates using anti-Phospho-eEF2 (pT56/58) RabMAb (Cat. #AJ1250d), 1:2000 dilution. Cells were either (A) untreated (B) starved.| Product Name | eEF2 Antibody Phospho (pT56/58) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T56/58 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP833Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EF2 |
|---|---|
| Molecular Weight(MW) | 95338 Da |
| Function | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr 56/58 of human eEF2 was used as immunogen. The antibody only detects eEF2 phosphorylated on Tyreonine 56/58. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 1938 |
|---|
Application
Western blot analysis on 293 cell lysates using anti-Phospho-eEF2 (pT56/58) RabMAb (Cat. #AJ1250d), 1:2000 dilution. Cells were either (A) untreated (B) starved.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
