Application
Western blot analysis of EGR1 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35ug/lane). EGR1 (arrow) was detected using the purified Pab.Application
EGR1Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EGR1Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
EGR1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of EGR1 Antibody (N-term)(Cat#AP2904a) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
EGR1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).| Product Name | EGR1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KROX24, ZNF225 |
|---|---|
| Alternative Names | EGR1 KROX24 ZNF225 Early growth response protein 1 AT225 Nerve growth factor-induced protein A Transcription factor ETR103 Transcription factor Zif268 Zinc finger protein 225 Zinc finger protein Krox-24 |
| Molecular Weight(MW) | 57507 Da |
| Function | Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation |
| Tissue Specificity | This EGR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~45 amino acids from the N-terminal region of human EGR1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 1958 |
|---|
Application
Western blot analysis of EGR1 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35ug/lane). EGR1 (arrow) was detected using the purified Pab.
Application
EGR1Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EGR1Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
EGR1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of EGR1 Antibody (N-term)(Cat#AP2904a) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
EGR1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
