Application
A. Western blot analysis on PC12 cell lysate using anti- Phospho-eIF-2alpha (pS51) RabMAb (Cat. #AJ1253a); dilution 1:1,000. A: non treated cells B: treated with NGF C: treated with Pervanadate D: treated with FBSApplication
B. Immunohistochemical analysis of paraffin-embedded human liver carcinoma using anti-Phospho-eIF-2alpha (pS51) RabMAb (Cat. #AJ1253a).Application
C. Dot Blot analysis using anti-Phospho eIF2alpha (pS51) (Cat. #AJ1253a) against eIF2alpha peptides (A) phosphorylated at Serine 51 and (B) non-phosphorylated peptide.| Product Name | eIF-2a Antibody Phospho (pS51) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E90 |
| Host Species | Rabbit |
| Tested Applications | WBIHCDB |
| IHC:1:50~1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIF2A |
|---|---|
| Alternative Names | EIF2S1 EIF2A Eukaryotic translation initiation factor 2 subunit 1 Eukaryotic translation initiation factor 2 subunit alpha |
| Molecular Weight(MW) | 36112 Da |
| Function | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a was used as immunogen. The antibody only detects eIF-2a phosphorylated on Serine 51. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology. |
| Cellular Localization | Cytoplasmic granule (By similarity). Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules (By similarity) |
| Entrez Gene | 1965 |
|---|
Application
A. Western blot analysis on PC12 cell lysate using anti- Phospho-eIF-2alpha (pS51) RabMAb (Cat. #AJ1253a); dilution 1:1,000. A: non treated cells B: treated with NGF C: treated with Pervanadate D: treated with FBS
Application
B. Immunohistochemical analysis of paraffin-embedded human liver carcinoma using anti-Phospho-eIF-2alpha (pS51) RabMAb (Cat. #AJ1253a).
Application
C. Dot Blot analysis using anti-Phospho eIF2alpha (pS51) (Cat. #AJ1253a) against eIF2alpha peptides (A) phosphorylated at Serine 51 and (B) non-phosphorylated peptide.| Application Notes | IHC:1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM231976