Application
Western blot analysis of EUHMTASE (arrow) using rabbit polyclonal EHMT1 (EUHMTASE1) Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EUHMTASE gene (Lane 2) (Origene Technologies).| Product Name | EHMT1 (EUHMTASE1) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EUHMTASE1, GLP, KIAA1876, KMT1D |
|---|---|
| Alternative Names | EHMT1 EUHMTASE1 GLP KIAA1876 KMT1D Histone-lysine N-methyltransferase EHMT1 Euchromatic histone-lysine N-methyltransferase 1 G9a-like protein 1 Histone H3-K9 methyltransferase 5 Lysine N-methyltransferase 1D |
| Molecular Weight(MW) | 141466 Da |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53 |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus. Chromosome. Note=Associates with euchromatic regions |
| Entrez Gene | 79813 |
|---|
Application
Western blot analysis of EUHMTASE (arrow) using rabbit polyclonal EHMT1 (EUHMTASE1) Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EUHMTASE gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
